Journal
BIOTECHNOLOGY LETTERS
Volume 25, Issue 22, Pages 1941-1944Publisher
KLUWER ACADEMIC PUBL
DOI: 10.1023/B:BILE.0000003990.27624.04
Keywords
genomic DNA; Magnaporthe grisea; microwave treatment; PCR; RAPD
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Genomic DNA was isolated from as little as 2 mg dry biomass of Magnaporthe grisea by microwave treatment within 30 s. The quantity of DNA was good enough for PCR analysis and Dot blot hybridization. This technique can be used for various studies, such as DNA fingerprinting to study the population structure of the phytopathogen in different regions, and for a quick screening of M. grisea transformants.
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