Journal
JOURNAL OF EXPERIMENTAL MEDICINE
Volume 198, Issue 11, Pages 1699-1706Publisher
ROCKEFELLER UNIV PRESS
DOI: 10.1084/jem.20031428
Keywords
B cell development; adaptor; signaling; proliferation; receptor
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SLP-65(-/-) pre-B cells show a high proliferation rate in vitro. We have shown previously that lambda5 expression and consequently a conventional pre-B cell receptor (pre-BCR) are essential for this proliferation. Here, we show that pre-B cells express a novel receptor complex that contains a L heavy chain (muHC) but lacks any surrogate (SL) or conventional light chain (LC). This SL-deficient pre-BCR (SL(-)pre-BCR) requires Ig-alpha for expression on the cell surface. Anti-mu treatment of pre-B cells expressing the SL(-)pre-BCR induces tyrosine phosphorylation of substrate proteins and a strong calcium (Ca2+) release. Further, the expression of the SL(-)pre-BCR is associated with a high differentiation rate toward kappaLC-positive cells. Given that B cell development is only partially blocked and allelic exclusion is unaffected in SL-deficient mice, we propose that the SL(-)pre-BCR is involved in these processes and therefore shares important functions with the conventional pre-BCR.
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