Journal
BIOTECHNOLOGY LETTERS
Volume 25, Issue 1, Pages 35-38Publisher
KLUWER ACADEMIC PUBL
DOI: 10.1023/A:1021717828907
Keywords
artemisinin; Artemisia annua L.; callus; cryopreservation; optimization
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Cryopreservation of callus tissue of Artimisia annua L. was optimized. Two lines of calli were precultured on MS medium with 5% (v/v) dimethyl sulfoxide, and protected by a cryoprotectant containing 15% (v/v) ethylene glycol, 15% (v/v) dimethyl sulfoxide, 30% (v/v) glycerol and 13.6% (w/v) sucrose. The highest survival rate of callus A201 reached 87% after it was pretreated at 25degreesC, cryopreserved by liquid nitrogen, recovered in water bath at 25degreesC and reloaded at 25degreesC with 34% (w/v) sucrose solution, and that of callus A202 reached 78% after it was treated as callus A201, except pretreated at 35degreesC, recovered at 35degreesC and reloaded with 47.8% (w/v) sucrose solution.
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