Journal
EUROPEAN JOURNAL OF IMMUNOLOGY
Volume 33, Issue 1, Pages 119-124Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/immu.200390014
Keywords
Annexin V; apoptosis; NK cell; cytotoxicity; IL-2 receptor
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Funding
- NCI NIH HHS [1R01-CA82016] Funding Source: Medline
- NIDCR NIH HHS [P01-1-DE12321] Funding Source: Medline
- NATIONAL INSTITUTE OF DENTAL &CRANIOFACIAL RESEARCH [P01DE012321] Funding Source: NIH RePORTER
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Natural killer (NK) cells (CD56(+)/CD3(-)) in the circulation of cancer patients were reported to have low NK activity and undergo spontaneous apoptosis. A possible relationship between apoptosis and impaired NK activity was studied by Annexin V-binding and NK-cell assays performed with peripheral blood mononuclear cells of patients with head and neck cancer (HNC), breast cancer (BC) and normal controls (NC). Cells stained with Annexin V (Anx) and antibodies to CD56, CD3, CD95, CD25, CD122 or CD132 were examined by flow cytometry. NK activity was tested against K562 targets in 4-h Cr-51-release assays. The ratio of CD56(dim)/CD56(bright) NK cells was significantly different in patients vs. controls (10 vs. 16; p<0.01). A significantly greater percentage of CD56(dim) NK cells bound Anx in HNC patients (27+/-17%, median SD) or BC (46+/-18%) than in NC (1 5+/-18%, p<0.04 and p<0.0002, respectively). CD56(dim) NK cells were preferentially targeted for apoptosis. NK activity was significantly lower in patients with HNC and BC than in NC (p<0.009). An inverse correlation between NK activity and the percent of Anx(+)CD56(dim) NK cells was observed in cancer patients (p =0.002) but not in NC. In patients, circulating CD56(dim) NK cells were targeted for apoptosis, leading to low levels of NK activity.
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