Journal
CELL BIOCHEMISTRY AND BIOPHYSICS
Volume 68, Issue 2, Pages 387-396Publisher
HUMANA PRESS INC
DOI: 10.1007/s12013-013-9719-0
Keywords
Hepatic stellate cell; Capsaicin; Proliferation; Migration; Activation
Funding
- CAPES/DGU of the Brazilian Ministry of Science and Technology [BEX 4426/10-0]
- Secretaria de Estado de Universidades of the Spanish Ministry of Science and Innovation [PHB2008-0080-PC]
- CAPES
- Vrije Universiteit Brussels
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Capsaicin, the active component of chili pepper, has been reported to have antiproliferative and anti-inflammatory effects on a variety of cell lines. In the current study, we aimed to investigate the effects of capsaicin during HSC activation and maintenance. Activated and freshly isolated HSCs were treated with capsaicin. Proliferation was measured by incorporation of EdU. Cell cycle arrest and apoptosis were investigated using flow cytometry. The migratory response to chemotactic stimuli was evaluated by a modified Boyden chamber assay. Activation markers and inflammatory cytokines were determined by qPCR, immunocytochemistry, and flow cytometry. Our results show that capsaicin reduces HSC proliferation, migration, and expression of profibrogenic markers of activated and primary mouse HSCs. In conclusion, the present study shows that capsaicin modulates proliferation, migration, and activation of HSC in vitro.
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