4.3 Article

Conformational Preferences of Modified Nucleoside N(4)-Acetylcytidine, ac4C Occur at Wobble 34th Position in the Anticodon Loop of tRNA

Journal

CELL BIOCHEMISTRY AND BIOPHYSICS
Volume 66, Issue 3, Pages 797-816

Publisher

HUMANA PRESS INC
DOI: 10.1007/s12013-013-9525-8

Keywords

tRNA; ac(4)C; Molecular electrostatic potentials (MEPS); RM1; MD simulation

Funding

  1. University Grants Commission, New Delhi
  2. UGC

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Conformational preferences of modified nucleoside, N(4)-acetylcytidine, ac(4)C have been investigated using quantum chemical semi-empirical RM1 method. Automated geometry optimization using PM3 method along with ab initio methods HF SCF (6-31G**), and density functional theory (DFT; B3LYP/6-31G**) have also been made to compare the salient features. The most stable conformation of N(4)-acetyl group of ac(4)C prefers proximal orientation. This conformation is stabilized by intramolecular hydrogen bonding between O(7)center dot center dot center dot HC(5), O(2)center dot center dot center dot HC2', and O4'center dot center dot center dot HC(6). The proximal conformation of N(4)-acetyl group has also been observed in another conformational study of anticodon loop of E. coli elongator tRNA(Met). The solvent accessible surface area (SASA) calculations revealed the role of ac(4)C in anticodon loop. The explicit molecular dynamics simulation study also shows the proximal orientation of N(4)-acetyl group. The predicted proximal conformation would allow ac(4)C to interact with third base of codon AUG/AUA whereas the 'distal' orientation of N(4)-acetyl cytidine side-chain prevents such interactions. Single point energy calculation studies of various models of anticodon-codon bases revealed that the models ac(4)C((34))(Proximal):G(3), and ac(4)C((34))(Proximal):A(3) are energetically more stable as compared to models ac(4)C((34))(Distal):G(3), and ac(4)C((34))(Distal):A(3), respectively. MEPs calculations showed the unique potential tunnels between the hydrogen bond donor-acceptor atoms of ac(4)C((34))(Proximal):G(3)/A(3) base pairs suggesting role of ac(4)C in recognition of third letter of codons AUG/AUA. The distal conformation of ac(4)C might prevent misreading of AUA codon. Hence, this study could be useful to understand the role of ac(4)C in the tertiary structure folding of tRNA as well as in the proper recognition of codons during protein biosynthesis process.

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