Journal
CELL BIOCHEMISTRY AND BIOPHYSICS
Volume 65, Issue 2, Pages 211-216Publisher
HUMANA PRESS INC
DOI: 10.1007/s12013-012-9422-6
Keywords
Time-varying magnetic field; Transmembrane molecular transport; Media conductivity; Cytotoxicity effect
Funding
- Student Research Committee of Tarbiat Modares University
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The cell membrane acts as a barrier that hinders free entrance of most hydrophilic molecules into the cell. Owing to the numerous applications, the introduction of non-permeate molecules into biologic cells has drawn considerable attention in the past years. The aim of our study was to investigate the effect of time-varying magnetic field on transmembrane molecular transport by measuring bleomycin cytotoxicity and conductivity modifying in K562 cells. The cells were exposed to magnetic pulses of 2.2 T strength peak and about 250-mu s duration via Magstim stimulator and double 70-mm coil. Three different frequencies of 0.25, 1, and 10 Hz pulses for 56,112, and 28 numbers of pulses, respectively, were applied (nine experimental groups) and uptake and conductivity was measured in each group. Our results show that time-varying magnetic field increase transmembrane molecular transport and media conductivity; this enhancement is greater for 28 pulses with 1 Hz frequency. The observed uptake enhancement due to magnetic exposure is considerable.
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