Journal
BIOTECHNOLOGY LETTERS
Volume 25, Issue 1, Pages 51-54Publisher
KLUWER ACADEMIC PUBL
DOI: 10.1023/A:1021730131633
Keywords
chiral alcohols; Lactobacillus brevis; NADH oxidase; oxidation
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A new NADH oxidase, useful for the regeneration of NAD+, was isolated and characterized from Lactobacillus brevis. In crude extracts the activity was from 10-15 U mg(-1). After purification by four chromatographic steps, an activity of 116 U mg(-1) was obtained with 14% yield. Highest activity was from pH 5.5-7 and at 40degreesC. The enzyme requires dithiothreitol to prevent oxidative deactivation. The K-m value for NADH was 24 muM.
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