Journal
CELL BIOCHEMISTRY AND BIOPHYSICS
Volume 56, Issue 2-3, Pages 115-124Publisher
HUMANA PRESS INC
DOI: 10.1007/s12013-009-9075-2
Keywords
Cell morphology; Cell alignment; Actin filaments; Polydimethylsiloxane; NIH 3T3 fibroblasts; Uniaxial strip stretch; Shear fluid flow
Funding
- National Science Foundation
- National Institute of Health
- Office of Naval Research
- Beckman Young Investigators Program
- Carnegie Mellon University, USA
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Cells are complex, dynamic systems that respond to various in vivo stimuli including chemical, mechanical, and scaffolding alterations. The influence of mechanics on cells is especially important in physiological areas that dictate what modes of mechanics exist. Complex, multivariate physiological responses can result from multi-factorial, multi-mode mechanics, including tension, compression, or shear stresses. In this study, we present a novel device based on elastomeric materials that allowed us to stimulate NIH 3T3 fibroblasts through uniaxial strip stretching or shear fluid flow. Cell shape and structural response was observed using conventional approaches such as fluorescent microscopy. Cell orientation and actin cytoskeleton alignment along the direction of applied force were observed to occur after an initial 3 h time period for shear fluid flow and static uniaxial strip stretching experiments although these two directions of alignment were oriented orthogonal relative to each other. This response was then followed by an increasingly pronounced cell and actin cytoskeleton alignment parallel to the direction of force after 6, 12, and 24 h, with 85% of the cells aligned along the direction of force after 24 h. These results indicate that our novel device could be implemented to study the effects of multiple modes of mechanical stimulation on living cells while probing their structural response especially with respect to competing directions of alignment and orientation under these different modes of mechanical stimulation. We believe that this will be important in a diversity of fields including cell mechanotransduction, cell-material interactions, biophysics, and tissue engineering.
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