Journal
CELL BIOCHEMISTRY AND BIOPHYSICS
Volume 53, Issue 1, Pages 33-42Publisher
HUMANA PRESS INC
DOI: 10.1007/s12013-008-9035-2
Keywords
Voltage-dependent ion channels; Activation; Inactivation; Action potential; Laser; Temperature
Funding
- Chinese National Science Foundations [30470416, 30870582]
- National 985'' Project
- U. S. NIH [REY016754A]
- American Heart Association [0665201Y]
- National Center for Research Resources [C06-RR-12088-01]
- NATIONAL CENTER FOR RESEARCH RESOURCES [C06RR012088] Funding Source: NIH RePORTER
- NATIONAL EYE INSTITUTE [R21EY016754] Funding Source: NIH RePORTER
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Optical control of neuronal activity has a number of advantages over electrical methods and can be conveniently applied to intact individual neurons in vivo. In this study, we demonstrated an experimental approach in which a focused continuous near-infrared (CNI) laser beam was used to activate single rat hippocampal neurons by transiently elevating the local temperature. Reversible changes in the amplitude and kinetics of neuronal voltage-gated Na and K channel currents were recorded following irradiation with a single-mode 980 nm CNI-laser. Using single-channel recordings under controlled temperatures as a means of calibration, it was estimated that temperature at the neuron rose by 14A degrees C in 500 ms. Computer simulation confirmed that small temperature changes of about 5A degrees C were sufficient to produce significant changes in neuronal excitability. The method should be broadly applicable to studies of neuronal activity under physiological conditions, in particular studies of temperature-sensing neurons expressing thermoTRP channels.
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