Journal
CELL AND TISSUE RESEARCH
Volume 374, Issue 3, Pages 541-553Publisher
SPRINGER
DOI: 10.1007/s00441-018-2894-y
Keywords
Cell dimensions; Microtissue; Cell spheroid; Bone marrow mesenchymal stromal cells; Tissue regeneration; BMP-2; Osteogenic induction
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Funding
- National Health and Medical Research Council (NHMRC) of Australia - NHMRC
- Prostate Cancer Foundation of Australia (PCFA)
- Ministry of Higher Education of Egypt
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Bone marrow-derived mesenchymal stem/stromal cells (BMSC) may facilitate bone repair through secretion of factors that stimulate endogenous repair processes or through direct contribution to new bone through differentiation into osteoblast-like cells. BMSC microtissue culture and differentiation has been widely explored recently, with high-throughput platforms making large-scale manufacture of microtissues increasingly feasible. Bone-like BMSC microtissues could offer an elegant method to enhance bone repair, especially in small-volume non-union defects, where small diameter microtissues could be delivered orthoscopically. Using a high-throughput microwell platform, our data demonstrate that (1) BMSC in 3D microtissue culture result in tissue compaction, rather than growth, (2) not all mineralised bone-like matrix is incorporated in the bulk microtissue mass and (3) a significant amount of lipid vacuole formation is observed in BMSC microtissues exposed to BMP-2. These factors should be considered when optimising BMSC osteogenesis in microtissues or developing BMSC microtissue-based therapeutic delivery processes.
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