Journal
CELL AND TISSUE RESEARCH
Volume 358, Issue 2, Pages 627-632Publisher
SPRINGER
DOI: 10.1007/s00441-014-1940-7
Keywords
Sirtuin 1; p53; NF-kappa B (p50/p65); Proliferation; Apoptosis; Porcine ovarian granulosa cells
Categories
Funding
- Ministry of Agriculture of the Slovak Republic [RUVV 07-13]
- Agency for Support of Research and Development of Slovak Republic (APVV) [APVV-0854-11, APVV-4040-11, 26220220176]
- Operational Programme Research and Development - European Regional Development Fund
- Deanship of Scientific Research at King Saud University [NoRGP-VPP-164]
Ask authors/readers for more resources
The roles of the mTOR system enzyme sirtuin 1 (SIRT1), the transcription factor p53 and the nuclear factor kappaB (NF-kappa B) and their interrelationships in the control of ovarian function have not been well studied. We examine, in vitro, the involvement of SIRT1, p53 and the p65 and p50 subunits of NF kappa B and their interrelationships in the control of the apoptosis and proliferation of porcine ovarian granulosa cells. Monolayers of primary granulosa cells were transfected with cDNA constructs encoding SIRT1, p53, p65 or p50 alone or were co-transfected with gene constructs for SIRT1 together with p53, p65 or p50. The accumulation of SIRT1, markers of proliferation (mitogen-activated protein kinase or extracellular-signal-regulated kinases 1,2) and a marker of apoptosis (caspase 3) was detected by immunocytochemistry. Transfection of cells with a SIRT1 gene construct alone promoted the accumulation of SIRT1 and decreased the accumulation of proliferation markers but did not affect the marker of apoptosis. Transfection of cells with gene constructs encoding p53, p50 or p65 decreased the expression of proliferation markers but not the apoptosis marker. Co-transfection of cells with SIRT1 cDNA changed the action of p65 on cell proliferation from inhibitory to stimulatory. SIRT1 overexpression induced the pro-apoptotic action of p53 and p50 but not of p65 constructs. Thus, SIRT1, p53 and NF-kappa B are involved in the control of both the proliferation and the apoptosis of ovarian cells. These novel data on the cross-talk between the mTOR/SIRT1 system and the transcription factors p53 and NF-kappa B show both the inhibitory (proliferation) and stimulatory (apoptosis) influences of SIRT1 on transcription factor action in ovarian cells.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available