Pseudomonas rhizosphaerae sp nov., a novel species that actively solubilizes phosphate in vitro

A bacterial strain (designated IH5(T)), isolated from rhizospheric soil of grasses growing spontaneously in Spanish soil, actively solubilized phosphates in vitro when bicalcium phosphate was used as a phosphorus source. This strain was Gram-negative, strictly aerobic, rod-shaped and motile. The strain produced catalase, but not oxidase. Cellulose, casein, starch, gelatin, aesculin and urea were not hydrolysed. Growth was observed with many carbohydrates as the carbon source. The main non-polar fatty acids detected were hexadecenoic acid (Cl 6: 1), hexadecanoic acid (C-16:0) and octadecenoic acid (C-18:1). The hydroxy fatty acids detected were 3-hydroxydecanoic acid (C-10:0 3-OH), 3-hydroxydodecanoic acid (C-12:0 3-OH) and 2-hydroxydoclecanoic acid (C-12:0 2-OH). Phylogenetic analysis of 16S rRNA indicated that this bacterium belongs to the genus Pseudomonas in the gamma-subclass of the Proteobacteria and that the closest related species is Pseudomonas graminis. The DNA G + C content was 61 mol%. DNA-DNA hybridization showed 23% relatedness between strain IH5(T) and P. graminis DSM 11363(T). Therefore, strain IH5(T) belongs to a novel species from the genus Pseudomonas, for which the name Pseudomonas rhizosphaerae sp. nov. is proposed (type strain, IH5(T) = LMG 21640(T) = CECT 5726(T)).