Journal
MAGNETIC RESONANCE IN MEDICINE
Volume 50, Issue 6, Pages 1120-1126Publisher
JOHN WILEY & SONS INC
DOI: 10.1002/mrm.10651
Keywords
amide proton exchange; mobile protein; peptide; magnetization transfer; 9L gliosarcoma
Funding
- NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING [R21EB002666, P41EB015909] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE [R01NS031490] Funding Source: NIH RePORTER
- NIBIB NIH HHS [P41 EB015909, EB02666] Funding Source: Medline
- NINDS NIH HHS [NS31490] Funding Source: Medline
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In this work we demonstrate that specific MR image contrast can be produced in the water signal that reflects endogenous cellular protein and peptide content in intracranial rat 9L gliosarcomas. Although the concentration of these mobile proteins and peptides is only in the millimolar range, a detection sensitivity of several percent on the water signal (molar concentration) was achieved. This was accomplished with detection sensitivity enhancement by selective radiofrequency (RF) labeling of the amide protons, and by utilizing the effective transfer of this label to water via hydrogen exchange. Brain tumors were also assessed by conventional T-1-weighted, T-2-weighted, and diffusion-weighted imaging. Whereas these commonly-used approaches yielded heterogeneous images, the new amide proton transfer (APT) technique showed a single well-defined region of hyperintensity that was assigned to brain tumor tissue. (C) 2003 Wiley-Liss, Inc.
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