Journal
CELL AND TISSUE BANKING
Volume 13, Issue 2, Pages 353-361Publisher
SPRINGER
DOI: 10.1007/s10561-011-9261-5
Keywords
Growth factor; Human amniotic membrane; ELISA; Freeze-drying; Tissue-powdering; Irradiation
Categories
Ask authors/readers for more resources
The aim of this work is to quantify the total protein and growth factors content in a tissue-suspension obtained from processed human amniotic membrane (hAM). hAM was collected, frozen, freeze dried, powdered and sterilized by c-irradiation. At each step of the process, samples were characterized for the total protein amounts by a Bradford protein assay and for the growth factor concentrations by ELISA test of the tissue suspensions. Frozen-hAM samples show higher release of total proteins and specific growth factors in the tissue suspension in comparison with freeze-dried hAM. We observed that even if the protein extraction is hindered once the tissue is dried, the powdering process allows a greater release in the tissue suspension of total proteins and growth factors after tissue re-solubilization in comparison with only the freeze-drying process (+91 +/- 13% for EGF, +16 +/- 4% for HGF, +11 +/- 5% for FGF, +16 +/- 9% for TGF-beta 1), and a greater release of EGF (85 +/- 10%) in comparison with only the freezing process, because proteins become much readily solubilized in the solution. According with these results, we describe a protocol to obtain a new sterile biological product from hAM tissue, with well-known effects of thermal, mechanical and physical processes on the total protein and grow factors contents.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available