Journal
JOURNAL OF SOL-GEL SCIENCE AND TECHNOLOGY
Volume 26, Issue 1-3, Pages 1141-1144Publisher
KLUWER ACADEMIC PUBL
DOI: 10.1023/A:1020727311786
Keywords
bacteria; bio-encapsulation; gelatin; beta-galactosidase; viability
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Whole E. coli bacteria have been trapped within silica gels obtained via the acidification of sodium silicate and silica nanoparticles solutions. Their beta-galactosidase enzymatic activity increases with time, suggesting that their membrane is partially lysed during the encapsulation process. Such a lysis can be greatly reduced when encapsulation is performed in the presence of gelatin. The biocatalytic activity of trapped bacteria remains almost constant for more than a week. Moreover bacteria trapped in such gels remain able to incorporate glucose, showing that their viability has been preserved.
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