Journal
CELL
Volume 158, Issue 2, Pages 339-352Publisher
CELL PRESS
DOI: 10.1016/j.cell.2014.05.036
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Funding
- National Institutes of Health [AI00880, AI082850]
- National Science Foundation Faculty Early Career Development Award [MCB-0845099]
- National Science Foundation Center for Theoretical Biological Physics
- Direct For Biological Sciences
- Div Of Molecular and Cellular Bioscience [1411884] Funding Source: National Science Foundation
- Direct For Mathematical & Physical Scien
- Division Of Physics [1308264] Funding Source: National Science Foundation
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During B lymphocyte development, immunoglobulin heavy-chain variable (V-H), diversity (D-H), and joining (J(H)) segments assemble to generate a diverse antigen receptor repertoire. Here, we have marked the distal V-H and D-H-J(H)-E-mu regions with Tet-operator binding sites and traced their 3D trajectories in proB cells transduced with a retrovirus encoding Tetrepressor-EGFP. We found that these elements displayed fractional Langevin motion (fLm) due to the viscoelastic hindrance from the surrounding network of proteins and chromatin fibers. Using fractional Langevin dynamics modeling, we found that, with high probability, D(H)J(H) elements reach a V-H elementwithin minutes. Spatial confinement emerged as the dominant parameter that determined the frequency of such encounters. We propose that the viscoelastic nature of the nuclear environment causes coding elements and regulatory elements to bounce back and forth in a spring-like fashion until specific genomic interactions are established and that spatial confinement of topological domains largely controls first-passage times for genomic interactions.
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