Journal
ANALYTICAL SCIENCES
Volume 19, Issue 1, Pages 49-53Publisher
JAPAN SOC ANALYTICAL CHEMISTRY
DOI: 10.2116/analsci.19.49
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MALDI-TOF mass spectrometry was used to detect intracellular molecules from a single intact cell on monolayers of other cells. Intracellular molecules, e.g., histamine, were gradually increased in a mouse bone marrow-derived mast cell by a maturation process. A single cell was captured by a microsuction pipette, and the mass spectra of intracellular histamine were measured directly. Finally, the time course of the intracellular molecular contents and the maturation stage from a single cell were estimated.
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