Axonally Synthesized ATF4 Transmits a Neurodegenerative Signal across Brain Regions

In Alzheimer's disease (AD) brain, exposure of axons to A beta causes pathogenic changes that spread retrogradely by unknown mechanisms, affecting the entire neuron. We found that locally applied Ab1-42 initiates axonal synthesis of a defined set of proteins including the transcription factor ATF4. Inhibition of local translation and retrograde transport or knockdown of axonal Atf4 mRNA abolished Ab-induced ATF4 transcriptional activity and cell loss. Ab1-42 injection into the dentate gyrus (DG) of mice caused loss of forebrain neurons whose axons project to the DG. Protein synthesis and Atf4 mRNA were upregulated in these axons, and coinjection of Atf4 siRNA into the DG reduced the effects of Ab1-42 in the forebrain. ATF4 protein and transcripts were found with greater frequency in axons in the brain of AD patients. These results reveal an active role for intraaxonal translation in neurodegeneration and identify ATF4 as a mediator for the spread of AD pathology.