Journal
CELL
Volume 153, Issue 1, Pages 206-215Publisher
CELL PRESS
DOI: 10.1016/j.cell.2013.02.024
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Funding
- Gordon Ross Fellowship
- NIH Training Grant
- Ruth L. Kirschstein NRSA Fellowship from the NIH [CA138126]
- NIH [GM065997, 10565784]
- Gordon and Betty Moore Foundation
- Beckman Institute
- National Research Council of Science & Technology (NST), Republic of Korea [k13008] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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The modular SCF (Skp1, cullin, and F box) ubiquitin ligases feature a large family of F box protein substrate receptors that enable recognition of diverse targets. However, how the repertoire of SCF complexes is sustained remains unclear. Real-time measurements of formation and disassembly indicate that SCFFbxw7 is extraordinarily stable, but, in the Nedd8-deconjugated state, the cullin-binding protein Cand1 augments its dissociation by one-million-fold. Binding and ubiquitylation assays show that Cand1 is a protein exchange factor that accelerates the rate at which Cul1-Rbx1 equilibrates with multiple F box protein-Skp1 modules. Depletion of Cand1 from cells impedes recruitment of new F box proteins to pre-existing Cul1 and profoundly alters the cellular landscape of SCF complexes. We suggest that catalyzed protein exchange may be a general feature of dynamic macromolecular machines and propose a hypothesis for how substrates, Nedd8, and Cand1 collaborate to regulate the cellular repertoire of SCF complexes.
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