Distribution of cholecystokinin-immunoreactive cells in the digestive tract of the larval teleost, ayu, Plecoglossus altivelis

The ontogenetic development of cholecystokinin-immunoreactive (CCK-IR) cells was studied in larval ayu, Plecoglossus altivelis. This species has a straight digestive tract during the larval phase. CCK-IR cells were present in all the larvae from the day of hatching (0 days after hatching, DAH). An immunoreaction to anti-trypsinogen antibody was also detected in the pancreas at this stage. The distribution pattern of the CCK-IR cells was quantified by recording the location of CCK-IR cells at 1, 16, and 76 DAH. Although the number of CCK-IR cells increased during development, the distribution pattern of CCK-IR cells did not change until 76 DAH. The CCK-IR cells were scattered throughout the midgut, with the exception of the regions adjacent to the pyloric and rectal sphincters. No CCK-IR cells were detected in the foregut or the hindgut. This distribution pattern differs from species with rotated digestive tracts, whose CCK-IR cells are only found in the anterior part of the midgut. CCK-IR cells seem to be located in regions where the ingested food is retained and thus can easily receive chemical signals from the food and the digestive process in order to control the release of the hormone. (C) 2003 Elsevier Science (USA). All rights reserved.