Journal
CELL
Volume 155, Issue 6, Pages 1233-1243Publisher
CELL PRESS
DOI: 10.1016/j.cell.2013.10.055
Keywords
-
Categories
Funding
- Swiss National Science Foundation
- European Molecular Biology Organization, and Marie Curie Actions
- Alexander von Humboldt Foundation and Marie Curie Actions
- Robert Crooks Stanley Fellowship at Stevens Institute of Technology
- European Research Council [309271-NPCAtlas]
- EMBL's mechanical workshop
Ask authors/readers for more resources
The nuclear pore complex (NPC) is a fundamental component of all eukaryotic cells that facilitates nucleocytoplasmic exchange of macromolecules. It is assembled from multiple copies of about 30 nucleoporins. Due to its size and complex composition, determining the structure of the NPC is an enormous challenge, and the overall architecture of the NPC scaffold remains elusive. In this study, we have used an integrated approach based on electron tomography, single-particle electron microscopy, and crosslinking mass spectrometry to determine the structure of a major scaffold motif of the human NPC, the Nup107 subcomplex, in both isolation and integrated into the NPC. We show that 32 copies of the Nup107 subcomplex assemble into two reticulated rings, one each at the cytoplasmic and nuclear face of the NPC. This arrangement may explain how changes of the diameter are realized that would accommodate transport of huge cargoes.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available