Analysis of levofloxacin in pharmaceutical preparations by high performance thin layer chromatography

A new simple, precise, rapid, and selective high-performance thin layer chromatography (HPTLC) method has been developed for the analysis of levofloxacin in pharmaceutical formulations. The method uses lamotrigine as an internal standard. The stationary phase was silica gel 60F(254) prewashed with methanol; water-methanol-n-butanol-ammonia solution 5 + 5 + 5 + 0.4 (v/v) was used as mobile phase. Detection and quantification were performed densitometrically at lambda = 298 nm. The linear range of the analysis was 0.8-3.0 mug and the percentage recovery was 99.90%.