Journal
JOURNAL OF IMMUNOLOGICAL METHODS
Volume 283, Issue 1-2, Pages 17-25Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jim.2003.07.003
Keywords
HIV; antibody; phage display; gp120; inhibitors; vaccines
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Funding
- NCI NIH HHS [N01-CO-12400] Funding Source: Medline
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Identification of broadly cross-reactive human monoclonal antibodies (mAbs) has major implications for development of vaccines, inhibitors and research tools. Here we describe a sequential antigen panning (SAP) methodology that may facilitate the selection of such antibodies. An HIV-specific antibody Fab (m18) was selected from a human Fab phage-display library by SAP against several recombinant soluble HIV envelope glycoproteins (Envs) and Env-sCD4 complexes. This Fab bound to a variety of recombinant soluble Envs (gp140s) from primary HIV isolates representing different clades, and inhibited cell fusion and virus entry mediated by Envs of primary HIV isolates. The methodology and the results may have implications for development of HIV vaccines and inhibitors, as well as for identification of antibodies to conserved epitopes on rapidly mutating viruses and cells. Published by Elsevier B.V.
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