Journal
BIORESOURCE TECHNOLOGY
Volume 86, Issue 2, Pages 105-115Publisher
ELSEVIER SCI LTD
DOI: 10.1016/S0960-8524(02)00165-7
Keywords
Eucalyptus grandis; steam explosion; dilute sulfuric acid; factorial design; alkali-soluble lignin; thermoanalysis; enzymatic hydrolysis
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Steam explosion of Eucalyptus grandis has been carried out under various pretreatment conditions (200-210 degreesC, 2-5 min) after impregnation of the wood chips with 0.087 and 0.175% (w/w) H2SO4. This study, arranged as a 21 factorial design, indicated that pretreatment temperature is the most critical variable affecting the yield of steam-treated fractions. Pretreatment of 0.175% (w/w) H2SO4-impregnated chips at 210 degreesC for 2 min was the best condition for hemicellulose recovery (mostly as xylose) in the water soluble fraction, reaching almost 70% of the corresponding xylose theoretical yield. By contrast, lower pretreatment temperatures of 200 degreesC were enough to yield steam-treated substrates from which a 90% cellulose conversion was obtained in 48 h, using low enzyme loadings of a Celluclast 1.5 1 plus Novozym 188 mixture (Novo Nordisk). Release of water-soluble chromophores was monitored by U-v spectroscopy and their concentration increased with pretreatment severity. The yield of alkali-soluble lignin increased at higher levels of acid impregnation and pretreatment temperatures. Thermoanalysis of these lignin fractions indicated a pattern of lignin fragmentation towards greater pretreatment severities but lignin condensation prevailed at the most drastic pretreatment conditions. (C) 2002 Elsevier Science Ltd. All rights reserved.
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