Journal
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
Volume 10, Issue 1, Pages 19-21Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/CDLI.10.1.19-21.2003
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Funding
- NIAID NIH HHS [R01 AI033144, AI33144] Funding Source: Medline
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [R01AI033144] Funding Source: NIH RePORTER
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We investigated the effect of multiple freeze-thaw cycles on mumps, measles, and rubella virus serum antibody levels with whole-virus immunoglobulin G enzyme-linked immunoassays. Fresh serum samples from nine healthy adult volunteers were divided into six sets of five aliquots each. Samples were taken through a total of 10 freeze-thaw cycles and stored at 4degreesC until assayed. Each assay measurement was done in replicates of five, and the mean value was reported. After completing 10 freeze-thaw cycles, we found no clinically or statistically significant effect on measured antibody levels and found no discernible detrimental effect on the ability to measure these antibodies by enzyme-linked immunoassays.
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