Journal
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
Volume 41, Issue 1, Pages 23-25Publisher
WALTER DE GRUYTER & CO
DOI: 10.1515/CCLM.2003.005
Keywords
peroxynitrite; 3-nitrotyrosine; atherosclerosis
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The effect of peroxynitrite on the development of atherosclerosis is one of the major foci of recent studies. Here, the cytotoxic effect of peroxynitrite was investigated by quantitatively measuring nitrated tyrosine, 3-nitrotyrosine (3-NT) levels in atherosclerotic blood vessels. Atherosclerotic vessels were obtained from the patients who underwent either coronary artery or peripheric artery bypass surgery. Internal thoracic arteries of the patients were treated as nonatherosclerotic control vessels. 3-NT was measured by reverse-phase HPLC and plasma nitrite-nitrate levels were measured by spectrophotometry. 3-NT levels were significantly elevated in atherosclerotic vessels (46.6+/-23.3 nmol/mg protein, n=15; p<0.001) in comparison to control vessels (15.8+/-2.5 nmol/mg protein, n=10). Vessel 3-NT correlated weakly with plasma nitrate levels (r=0.38). Thus, atherosclerotic arteries have higher 3-NT levels than nonatherosclerotic blood vessels.
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