Journal
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
Volume 26, Issue 9-10, Pages 1659-1669Publisher
MARCEL DEKKER INC
DOI: 10.1081/JLC-120021274
Keywords
lutein; countercurrent chromatography; carotenoids; marigold flower petal
Ask authors/readers for more resources
Analytical high-speed countercurrent chromatography (HSCCC) was used for the systematic selection and optimization of the two-phase solvent system to separate lutein from the crude extract of the marigold flower petal. Lutein was obtained by preparative HSCCC with a two-phase solvent system composed of n-heptane-chloroform-acetonitrile (10 : 3: 7, v/v/v). HPLC analyses of the lutein revealed that it was over 98.5% purity. The final identification was performed by UV-VIS absorption, H-1-NMR, and C-13-NMR Spectra.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available