Journal
CELL
Volume 135, Issue 3, Pages 475-485Publisher
CELL PRESS
DOI: 10.1016/j.cell.2008.08.031
Keywords
-
Categories
Funding
- Agence Nationale de la Recherche
- Action Concertee Incitative (ACI) Informatique, Mathematiques, Physique en Biologie moleculaire
- ACI Biologie Cellulaire, Moleculaire, Structurale
- Association pour la Recherche sur le Cancer
Ask authors/readers for more resources
The organization of the Escherichia coli chromosome into insulated macrodomains influences the segregation of sister chromatids and the mobility of chromosomal DNA. Here, we report that organization of the Terminus region (Ter) into a macrodomain relies on the presence of a 13 bp motif called matS repeated 23 times in the 800-kb-long domain. matS sites are the main targets in the E. coli chromosome of a newly identified protein designated MatP. MatP accumulates in the cell as a discrete focus that colocalizes with the Ter macrodomain. The effects of MatP inactivation reveal its role as main organizer of the Ter macrodomain: in the absence of MatP, DNA is less compacted, the mobility of markers is increased, and segregation of Ter macrodomain occurs early in the cell cycle. Our results indicate that a specific organizational system is required in the Terminus region for bacterial chromosome management during the cell cycle.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available