Journal
CELL
Volume 134, Issue 3, Pages 521-533Publisher
CELL PRESS
DOI: 10.1016/j.cell.2008.07.020
Keywords
-
Categories
Funding
- NIH [5-RO1-HDO45022, 5-R37-CA084198, 5-RO1-CA087869, HG002668]
- Whitehead Institute
- Cancer Center Support (Core)
- NCI [P30-CA14051]
Ask authors/readers for more resources
MicroRNAs (miRNAs) are crucial for normal embryonic stem (ES) cell self-renewal and cellular differentiation, but how miRNA gene expression is controlled by the key transcriptional regulators of ES cells has not been established. We describe here the transcriptional regulatory circuitry of ES cells that incorporates protein-coding and miRNA genes based on high-resolution ChIP-seq data, systematic identification of miRNA promoters, and quantitative sequencing of short transcripts in multiple cell types. We find that the key ES cell transcription factors are associated with promoters for miRNAs that are preferentially expressed in ES cells and with promoters for a set of silent miRNA genes. This silent set of miRNA genes is co-occupied by Polycomb group proteins in ES cells and shows tissue-specific expression in differentiated cells. These data reveal how key ES cell transcription factors promote the ES cell miRNA expression program and integrate miRNAs into the regulatory circuitry controlling ES cell identity.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available