Journal
BIOCHEMISTRY-MOSCOW
Volume 68, Issue 11, Pages 1200-1209Publisher
MAIK NAUKA/INTERPERIODICA
DOI: 10.1023/B:BIRY.0000009134.48246.7e
Keywords
Penicillium canescens; endo-beta-1,4-xylanase; alpha-L-arabinofuranosidase; arabinoxylan-arabinofuranohydrolase; endo-beta-1,3/1,4-glucanase; chromatofocusing
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The composition of the enzyme complex secreted by Penicillium canescens was investigated. A scheme for purification of the main components of the complex by chromatofocusing on a Mono P column was developed. It was found that along with beta-galactosidase, the major components of the complex were endo-beta-1,4-xylanase (31 kD, pI 8.2-9.3), alpha-L-arabinofuranosidase (60 kD, pI 7.6), arabinoxylan-arabinofuranohydrolase (70 kD, pI 3.8-4.0), and endo-beta-1,3/1,4-glucanase (40 kD, pI 4.4). The substrate specificity, pH and temperature activity optima, adsorbability, thermal stability, and ability for synergic interaction of the isolated enzymes were studied.
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