Journal
CELL
Volume 134, Issue 6, Pages 995-1006Publisher
CELL PRESS
DOI: 10.1016/j.cell.2008.07.022
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Funding
- U.S. DOE [DE-AC02-05CH11231]
- NSLS [DE-AC02-98CH10886]
- American Lebanese Syrian Associated Charities of St. Jude,
- NIH [P30CA021765, R01GM069530]
- Howard Hughes Medical Institute
- Beckman Young Investigator Award
- Pew Scholar Award
- Phillip and Elizabeth Gross Foundation
- DOD [DAMD17-03-0420]
- American Cancer Society
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Cullin-RING ligases (CRLs) comprise the largest ubiquitin E3 subclass, in which a central cullin subunit links a substrate-binding adaptor with an E2-binding RING. Covalent attachment of the ubiquitin-like protein NEDD8 to a conserved C-terminal domain (ctd) lysine stimulates CRL ubiquitination activity and prevents binding of the inhibitor CAND1. Here we report striking conformational rearrangements in the crystal structure of NEDD8 similar to CuI5(ctd)-Rbx1 and SAXS analysis of NEDD8 similar to CuI1(ctd)-Rbx1 relative to their unmodified counterparts. In NEDD8ylated CRL structures, the cullin WHB and Rbx1 RING subdomains are dramatically reoriented, eliminating a CAND1-binding site and imparting multiple potential catalytic geometries to an associated E2. Biochemical analyses indicate that the structural malleability is important for both CRL NEDD8ylation and subsequent ubiquitination activities. Thus, our results point to a conformational control of CRL activity, with ligation of NEDD8 shifting equilibria to disfavor inactive CAND1-bound closed architectures, and favor dynamic, open forms that promote polyubiquitination.
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