Journal
CELL
Volume 132, Issue 5, Pages 860-874Publisher
CELL PRESS
DOI: 10.1016/j.cell.2008.02.020
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Funding
- Medical Research Council [MC_U120027516] Funding Source: Medline
- NIAID NIH HHS [AI-060302] Funding Source: Medline
- MRC [MC_U120027516] Funding Source: UKRI
- Medical Research Council [MC_U120027516] Funding Source: researchfish
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To explore the role of Dicer-dependent control mechanisms in B lymphocyte development, we ablated this enzyme in early B cell progenitors. This resulted in a developmental block at the pro- to pre-B cell transition. Gene-expression profiling revealed a miR-17 similar to 92 signature in the 3'UTRs of genes upregulated in Dicer-deficient pro-B cells; a top miR-17 similar to 92 target, the proapoptotic molecule Bim, was highly upregulated. Accordingly, B cell development could be partially rescued by ablation of Bim or transgenic expression of the prosurvival protein Bcl-2. This allowed us to assess the impact of Dicer deficiency on the V(D)J recombination program in developing B cells. We found intact Ig gene rearrangements in immunoglobulin heavy (IgH) and kappa chain loci, but increased sterile transcription and usage of D-H elements of the DSP family in IgH, and increased N sequence addition in Ig kappa due to deregulated transcription of the terminal deoxynucleotidyl transferase gene.
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