Journal
PLANT CELL REPORTS
Volume 22, Issue 4, Pages 274-281Publisher
SPRINGER
DOI: 10.1007/s00299-003-0691-9
Keywords
Agrobacterium; canola; infiltration; in planta
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We report here an in planta method to produce transgenic Brassica napus plants. The procedure included Agrobacterium-mediated inoculation of plants at various development stages along with a vacuum infiltration step. The flowering stage appeared to be the most receptive stage for transformation and production of transgenic plants. In some cases, the flowering stage was induced either by cold treatment or by high density planting. Molecular and genetic analysis revealed that single and multiple copy events were generated and that the transgenes were transmitted to the T-1 and T-2 progeny in a Mendelian fashion.
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