Journal
CELL CYCLE
Volume 2, Issue 2, Pages 143-148Publisher
TAYLOR & FRANCIS INC
DOI: 10.4161/cc.2.2.336
Keywords
Ask1; Dam1; Duo1; DASH; Cdc28; Phosphorylation; Mitosis
Categories
Funding
- National Institute of Health [GM44664]
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R37GM044664, R01GM044664] Funding Source: NIH RePORTER
Ask authors/readers for more resources
The proper timing and fidelity of cell cycle transitions is critical for the survival of organisms. Cyclin-dependent kinases orchestrate many cell cycle transitions in eukaryotes including S phase entry and mitosis. Accurate chromosome segregation during mitosis is one of the key events regulated by the cell cycle and many proteins function together to ensure the fidelity of this process. In S. cerevisiae, the DASH complex is essential for chromosome segregation. The DASH complex binds to microtubules and kinetochores and regulates their association. Here we report that Ask1, one component of DASH, is phosphorylated during the cell cycle. This phosphorylation is dependent on Cdks in vivo, and in vitro Cdc28 can phosphorylate Ask1. We identify two Cdk phosphorylation sites in Ask1 and find that the phosphorylation of Ask1 is important for its full activity in vivo. Thus, the DASH complex is directly regulated by cyclin-dependent kinases to facilitate chromosome segregation.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available