Journal
STRUCTURE
Volume 11, Issue 1, Pages 13-19Publisher
CELL PRESS
DOI: 10.1016/S0969-2126(02)00910-3
Keywords
protein crystals; radiation damage; synchrotron radiation; X-ray crystallography
Ask authors/readers for more resources
Is radiation damage to cryopreserved protein crystals strictly proportional to accumulated dose at the high-flux density of beams from undulators at third-generation synchrotron sources? The answer is yes, for overall damage to several different kinds of protein crystals at flux densities up to 10(15) ph/sec/mm(2) (APS beamline 19-ID). We find that, at 12 keV (1 Angstrom wavelength), about ten absorbed photons are sufficient to kill a unit cell. As this corresponds to about one elastically scattered photon, each unit cell can contribute only about one photon to total Bragg diffraction. The smallest crystal that can yield a full data set to 3.5 Angstrom resolution has a diameter of about 20 mum (100 Angstrom unit cell).
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available