Journal
JOURNAL OF PROTEOME RESEARCH
Volume 3, Issue 1, Pages 112-119Publisher
AMER CHEMICAL SOC
DOI: 10.1021/pr0340431
Keywords
isoelectric focusing; mass spectrometry; protein identification; proteomics; peptides
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Here we present the theoretical and experimental evaluation of peptide isoelectric point as a method to aid in the identification of peptides from complex mixtures. Predicted p/values were found to match closely the experimentally obtained data, resulting in the development of a unique filter that lowers the effective false positive rate for peptide identification. Due to the reduction of the false positive rate, the cross-correlation parameters X-corr and DeltaCn from the SEQUEST program can be lowered resulting in 25% more peptide identifications. This approach was successfully applied to analysis of the soluble fraction of the E. coli proteome, where 417 proteins were identified from 1022 peptides using just 20 mug of material.
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