Journal
GENOME BIOLOGY
Volume 5, Issue 10, Pages -Publisher
BMC
DOI: 10.1186/gb-2004-5-10-r81
Keywords
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Funding
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R43GM064036] Funding Source: NIH RePORTER
- NIGMS NIH HHS [R43 GM64036] Funding Source: Medline
- PHS HHS [R01 45335] Funding Source: Medline
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We have developed a screening technology for the identification of short-lived proteins. A green fluorescent protein (GFP)-fusion cDNA library was generated for monitoring degradation kinetics. Cells expressing a subset of the GFP-cDNA expression library were screened to recover those in which the fluorescence signal diminished rapidly when protein synthesis was inhibited. Thirty clones that met the screening criteria were characterized individually. Twenty-three (73%) proved to have a half-life of 4 hours or less.
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