Journal
JOURNAL OF MASS SPECTROMETRY
Volume 39, Issue 1, Pages 38-42Publisher
WILEY
DOI: 10.1002/jms.547
Keywords
atmospheric pressure chemical ionization; liquid chromatography/tandem mass spectrometry; lipid peroxidation; biomarker; DNA adducts; malondialdehyde
Funding
- NATIONAL CANCER INSTITUTE [P01CA077839] Funding Source: NIH RePORTER
- NCI NIH HHS [CA77839] Funding Source: Medline
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The major adduct of malondialdehyde with guanine, M(1)G, was measured in human urine from nonsmoking healthy individuals. M(1)G is a mutagenic DNA lesion and a terminal product of lipid peroxidation in vivo that may be implicated in cancer related to lifestyle and diet. On the basis of a recently developed method for the quantification of M(1)G as an excreted deoxynucleoside using immuno-extraction purification, chemical NaBH4 reduction and liquid chromatography combined with atmospheric pressure chemical ionization tandem mass spectrometry, we demonstrate that the average 24 h excretion rate of M(1)G-dR is about 12+/-3.8 fmol kg(-1) (n = 5). Copyright (C) 2004 John Wiley Sons, Ltd.
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