Journal
BIOFACTORS
Volume 21, Issue 1-4, Pages 113-117Publisher
WILEY
DOI: 10.1002/biof.552210122
Keywords
antioxidants; inflammation; macrophages; inflammatory cytokines; COX-2; iNOS
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Oxidative stresses are involoved in the process of chronic inflammatory diseases. The objective of this study was to evaluate and compare the anti-inflammatory activity of major dietary antioxidants. Murine RAW264.7 macrophages (4 X 10(6) cells) were incubated in DMEM containing 10% FBS supplemented with 5 to 20 muM of alpha-tocopherol, beta-carotene or quercetin for 24 hrs, and then treated with LPS for 16hrs. The medium was collected to measure TNF-alpha, IL-6, PGE(2) and nitrite. Expressions of cyclooxygenase-2(COX-2) and inducible nitric oxide synthase(iNOS) were determined using the harvested cells. Results indicate that TNF-alpha and IL-6 accumulations were significantly reduced by 5 to 20 muM quercetin treatment, and 20 muM of alpha-tocopherol treatment. Nitrite release was significantly reduced by 5 muM quercetin treatment. However, PGE(2) accumulation was not affected by any of the antioxidants used. Expressions of COX-2 and iNOS were effectively reduced by 5 muM quercetin treatment. These findings indicate that dietary antioxidants possess significant anti-inflammatory activities, and quercetin is the most potent antioxidant.
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