Journal
JOURNAL OF DENTAL RESEARCH
Volume 83, Issue 1, Pages 40-44Publisher
SAGE PUBLICATIONS INC
DOI: 10.1177/154405910408300108
Keywords
hyaluronan; HAS; synovial membrane; TMJ; transforming growth factor
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Hyaluronan (HA) synthesis in the synovial membrane is affected by various chemical mediators. It is hypothesized that transforming growth, factor-betal (TGF-beta1) would be a mediator to modulate HA synthesis in cultured synovial membrane fibroblasts of the temporomandibular joint (TMJ). Fibroblasts were extracted from the TMJ synovial membrane of four-week-old Japanese white rabbits. The amount of HA and expression levels of HA synthase (HAS) mRNAs induced by TGF-beta1 treatment were analyzed by means of high-performance liquid chromatography and real-time polymerase chain-reaction, respectively. Both medium and large Amounts of HA were enhanced by the stimulation of TGF-beta1. HAS2 mRNA expression was enhanced 13-fold after six-hour stimulation with TGF-beta1 (10 ng/mL), whereas HAS3 mRNA expression was not, changed significantly. These results suggest that TGF-beta1 enhances the expression of HAS2 mRNA in the TMJ synovial membrane fibroblasts and may contribute to the production of high-molecular-weight HA in the joint fluid.
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