Journal
MICROSCOPY RESEARCH AND TECHNIQUE
Volume 63, Issue 1, Pages 18-22Publisher
WILEY
DOI: 10.1002/jemt.10423
Keywords
confocal; second harmonic; multiphoton; resolution; super-resolution
Categories
Ask authors/readers for more resources
Calculated and measured resolution figures are presented for confocal microscopes with different pinhole sizes and for nonlinear (2-photon and second harmonic) microscopes. A modest degree of super-resolution is predicted for a confocal microscope but in practice this is not achievable and confocal fluorescence gives little resolution improvement over widefield. However, practical non-linear microscopes do approach their theoretical resolution and therefore show no resolution disadvantage relative to confocal microscopes in spite of the longer excitation wavelength.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available