Journal
ACTA BIOCHIMICA ET BIOPHYSICA SINICA
Volume 36, Issue 1, Pages 42-46Publisher
OXFORD UNIV PRESS
DOI: 10.1093/abbs/36.1.42
Keywords
plasminogen activator inhibitor type-2; proteasome (prosome, macropain) subunit, beta type 1(PSM beta 1); yeast two-hybrid system; ubiquitin
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The apoptosis protection by plasminogen activator inhibitor-2(PAI-2) is dependent on a 33 amino acid fragment between helix C and D of PAI-2 which is probably due to the interaction of PAI-2 with unknown intracellular proteins. In this study, we used the fragment between helix C and D of PAI-2 as bait to screen a HeLa cell cDNA library constructed during apoptosis in a yeast two-hybrid system and retrieved a clone encoding 241 amino acids of proteasome (prosome, macropain) subunit, beta type 1 (PSMbeta1) which plays important roles in NF-kappaB activation. GST-pulldown experiments confirmed the interaction between PAI-2 and PSMbeta1 in vitro. These data suggest that the antiapoptosis activity of PAI-2 is probably related to its interation with PSMbeta1.
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