Jasmonic acid and salicylic acid induce accumulation of beta-1,3-glucanase and thaumatin-like proteins in wheat and enhance resistance against Stagonospora nodorum

The effect of application of jasmonic acid (JA) and salicylic acid ( SA) on the induction of resistance in wheat to Stagonospora nodorum and on the induction of beta-1,3-glucanase and thaumatin-like proteins (TLPs) was studied. Western blot analysis revealed that two beta-1,3-glucanases with apparent molecular masses of 31 and 33 kDa that cross-reacted with a barley glucanase antiserum were induced in wheat leaves after treatment with JA and SA. When wheat plants were treated with SA and JA, a TLP with an apparent molecular mass of 25 kDa and several other isoforms of TLP were induced. Pre-treatment of wheat plants with SA and JA significantly reduced ( up to 56 %) the incidence of leaf blotch disease incited by S. nodorum compared with untreated control plants.