Journal
MICROBIOLOGY AND IMMUNOLOGY
Volume 48, Issue 11, Pages 889-892Publisher
WILEY
DOI: 10.1111/j.1348-0421.2004.tb03607.x
Keywords
clostridium difficile; toxin B; PCR; healthy human adult
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Prevalence rate of Clostridium difficile in healthy human adults is believed to be very low. Our RT-PCR system using glass powder, which can eliminate PCR inhibitors, detected C. difficile toxin B mRNA in 16 of 30 fecal samples (53.3%) from healthy human adults. In contrast, we failed to detect toxin B in the same fecal samples by PCR using DNA templates extracted with phenol-chloroform. Our results suggest that PCR inhibitors in feces carried through phenol-chloroform extraction procedure might suppress the sensitivity of PCR and that C. difficile is actually present in human gut microbiota more frequently than previously suspected.
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