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A fluorescent intercalator displacement assay for establishing DNA binding selectivity and affinity

Journal

ACCOUNTS OF CHEMICAL RESEARCH
Volume 37, Issue 1, Pages 61-69

Publisher

AMER CHEMICAL SOC
DOI: 10.1021/ar030113y

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Funding

  1. NCI NIH HHS [CA41986, CA78045] Funding Source: Medline
  2. NATIONAL CANCER INSTITUTE [R01CA041986, P01CA078045] Funding Source: NIH RePORTER

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A summary of the qualitative and quantitative elements of a fluorescent intercalator displacement (FID) assay useful for establishing the DNA binding selectivity, affinity, stoichiometry, and binding site size and distinguishing modes of DNA binding is provided.

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