Journal
JOURNAL OF MOLECULAR NEUROSCIENCE
Volume 22, Issue 3, Pages 167-177Publisher
HUMANA PRESS INC
DOI: 10.1385/JMN:22:3:167
Keywords
autoradiography; caudate putamen; dopamine receptors; D-4-receptor; genetic knockout; glutamate receptors; mutant mice
Categories
Ask authors/readers for more resources
Expression of dopamine ([DA] D-1 and D-2) and glutamate ([Glu], (N-methyl-D-aspartic acid [NMDA] alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid [AMPA], and kanaic acid [KA]) receptor types were analyzed autoradiographically in forebrain regions of D-4 receptor knockout mice and their wild-type controls. Selective radioligand binding to D-4 receptors was virtually absent in D-4 receptor knockout mouse brain in contrast to significant specific D-4 binding in forebrain tissue of wild-type controls. Labeling of D-1 receptors was significantly increased in nucleus accumbens (NAc; 39%) and caudate putamen (CPu; 42%) of D-4-knockout mice vs wild-type controls. In addition, NMDA receptor labeling was significantly increased in NAc (31%), CPu (40%), and hippocampal CA(1) (21%) and CA(3) (25%) regions of D-4 knockouts vs wild-type controls. No changes in D-2, AMPA or KA receptors were found. The findings suggest that D-1, D-4, and NMDA receptors might interact functionally and that developmental absence of D-4 receptors might trigger compensatory mechanisms that enhance expression of D-1 receptors in NAc and CPu, and NMDA receptors in NAc, CPu, and hippocampus. The findings also encourage cautious interpretation of results in knockout mice with targeted absence of specific genes, as complex adaptive changes not directly related to the missing gene might contribute to physiological and behavioral responses.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available