Journal
EUROPEAN JOURNAL OF MASS SPECTROMETRY
Volume 10, Issue 5, Pages 657-664Publisher
SAGE PUBLICATIONS LTD
DOI: 10.1255/ejms.671
Keywords
Duchenne muscular dystrophy; glycerophosphatidylcholine; fatty acid; imaging mass spectrometry; MALDI-ToF
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Duchenne muscular dystrophy (DMD) is a neuromuscular disease linked to the lack of dystrophin, a submembrane protein, leading to muscle weakness and associated with a defect of the lipid metabolism. A study of the fatty acid composition of glycerophosphatidylcholines by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-ToF-MS) and tandem mass spectrometry (MS/MS) enabled us to characterize a change in the lipid composition of dystrophic cells at the time of the differentiation. This modification has been used as a marker to identify with profiling and imaging MALDI-ToF MS regenerating areas in sections of an mdx mouse leg muscle. It is the first time that such a slight change in fatty acid composition has been observed directly on tissue slices using mass spectrometry. This approach will be useful in monitoring the treatment of muscular regeneration.
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