The mediating role of cPLA(2) in IL-1 beta and IL-6 release in LPS-induced HeLa cells

Studies were conducted to characterize a HeLa cell model by which the roles of the 85-kDa phospholipase A(2) (cPLA(2)) in interleukin-1 beta (IL-I beta) and interleukin-6 (IL-6) release could be evaluated. At first, untreated HeLa cells were compared with lipopolysaccharide (LPS)-treated HeLa cells. The latter resulted in cPLA(2) overexpression and an increased trend of IL-1 beta and IL-6 release. The indicated doses of 85-kDa cPLA(2) antisense oligonucleotide directed against the initiation site were then used to block cPLA(2) in LPS-induced HeLa cells. The process led to a dose-dependent decrease in cPLA(2) protein with no noticeable change of cPLA(2) mRNA. Compared with that of LPS added only, a reduction of IL-1 beta and IL-6 levels in the supernatants of transfected cells following the repression of cPLA(2) was observed. These results suggested that 85-kDa cPLA(2) may mediate the signalling cascades by which IL-1 beta and IL-6 were released in LPS-induced HeLa cells. Copyright (C) 2003 John Wiley Sons, Ltd.