Journal
TUBERCULOSIS
Volume 84, Issue 6, Pages 375-385Publisher
CHURCHILL LIVINGSTONE
DOI: 10.1016/j.tube.2004.05.001
Keywords
Mycobacterium tuberculosis; IP-10; IFN gamma; IL-12; TGF beta; IL-10; IL-4; immunohistochemistry
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Setting: Following infection with Mycobacterium tuberculosis, host cytokine responses influence disease manifestation. Differences in cytokine expression likely determine whether tuberculosis (TB) progresses, resolves, or becomes Latent. In particular, the balance between Th-1 and Th-2 cytokine responses influences the expression of disease in individuals with pulmonary TB. Objective and Design: Since the cytokine microenvironment in pulmonary TB remains suboptimally defined, we utilized quantitative immunohistochemistry to compare the expression of Th-1 cytokines [interferon-gamma (IFNgamma) and interleukin-12 (IL-12)] and Th-2 cytokines [IL-4, IL-10, transforming growth factor-beta (TGFbeta)] in surgically resected Lungs of seven TB patients and four control subjects. We also quantified IFNgamma-inducible protein 10 (IP-10) expression, a CXC chemokine for macrophages; and T cells. Results: Morphometric analyses revealed increased IFNgamma, IL-12, IP-10, and TGFbeta in granulomas and in pneumonitis areas of TB lungs. In contrast, IL-10 and IL-4 expressions were globally reduced in TB lung tissues compared to controls. Conclusion: Th-1 cytokines and TGFbeta are increased while Th-2 cytokines are decreased in well-formed pulmonary granulomas of TB patients compared to controls. (C) 2004 Elsevier Ltd. All rights reserved.
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